Analytical Chemistry (2023). DOI: 10.1021/acs.analchem.3c00573″> This illustration shows how the nanopore filter made with the α-hemolysin protein prevents sample contamination and facilitates single-molecule counting of DNA molecules. Credit: Analytical Chemistry (2023). DOI: 10.1021/acs.analchem.3c00573
Single-molecule DNA detection using nanopore technology offers real-time analysis of DNA and RNA strands. It is a low-cost and flexible technique that can be used in clinical and research settings when samples need to be analyzed quickly and efficiently. However, this is new technology that still has some drawbacks, such as sample contamination.
Research to improve and optimize single-molecule DNA extraction with nanopores is ongoing. A recently published paper outlines how a DNA-filtering system using a nanopore developed from the α-hemolysin protein.
The paper was published on June 6 in Analytical Chemistry.
“We set out to develop a DNA filtering system using α-hemolysin (αHL) nanopores